Advanced diagnostic, therapeutic, and transport applications of antibodies
Antibodies have become widely used agents for clinical treatment of cancers and autoimmune diseases due to their high level of specificity and selectivity toward known target molecules (antigens) found to be overexpressed on cancerous cells. Despite the many techniques that utilize antibodies necessitate that they are modified in some way by conjugating functional ligands to them – endowing them with specific functionalities unique for each intended application – they are still in need of improvement.
This talk will be focusing on addressing the particular technical challenges related with site-specific labelling of antibodies in order to enhance diagnostic and therapeutic platforms, specifically focusing on the great challenge in the field “how to cross the blood-brain barrier (BBB)”. Therefore, the talk will cover three major research subjects: i) development of advanced diagnostic methods and tools; ii) efficient, very affordable and indestructible affinity column methods for antibody purification; and iii) development of screening tools for identifying shuttle molecules that can cross the BBB. In the first part, I will be introducing UV-NBS method for oriented immobilization of antibodies and their Fab fragments to small scaled diagnostic surfaces, such as microfluidic devices andnanoparticles, while preserving their antigen binding activity and structure stability. In the second part, I will be talking about designing a novel affinity chromatography technique for purification of antibodies from complex protein environment that is an easy, efficient and cost-effective way. In the final part, I will be describing an enhanced human BBB model created with microfluidic Organ Chip culture technology that contains human iPS-derived brain microvascular endothelial cells (BMVEC) interfaced with primary human pericytes and astrocytes, and that uses a developmentallyinspired differentiation protocol, as well as provide examples for its usage for drug development and new therapeutic discoveries.
About The Speaker
Dr. Mustafaoglu has graduated from Istanbul Technical University in 2010, and she holding BSc degrees from Molecular Biology and Genetics Department and Physical Engineering Department. She earned her PhD degree from University of Notre Dame, IN, USA, in 2017. She has worked on developing drug conjugates, biomaterials, (nano)biosensors for health applications by incorporating and engineering antibody fragments. She has started a postdoctoral position in Spetember 2017 at Harvard University Wyss Institute for Biologically Inspired Engineering. Currenely she is working on bioinspired therapeutics and diagnostics”