Ambient-Temperature Serial Femtosecond X-ray Crystallographic Studies of Ribosome Complexes
High-resolution ribosome structures determined by cryo X-ray crystallography have provided important insights into the mechanism of translation. Such studies have thus far relied on large ribosome crystals kept at cryogenic temperatures to reduce radiation damage. Here I will describe the application of serial femtosecond X-ray crystallography (SFX) using an X-ray free-electron laser (XFEL) to obtain diffraction data from ribosome microcrystals in liquid suspension at ambient temperature. Small 30S ribosomal subunit microcrystals programmed with decoding complexes and bound to either antibiotic compounds or their next-generation derivatives diffracted to high resolution. Our results demonstrate the feasibility of using SFX to better understand the structural mechanisms underpinning the interactions between ribosomes and other substrates such as antibiotics and decoding complexes. We have also determined the structure of large (50S) ribosomal subunit in record-short time by using record-low amount of sample during and XFEL beamtime . This structure is the largest one solved to date by any FEL source to near atomic resolution (3 MDa). We expect that these results will enable routine structural studies, at near-physiological temperatures, of the large ribosomal subunit bound to clinically-relevant classes of antibiotics targeting it, e.g. macrolides and ketolides, also with the goal of aiding development of the next generation of these classes of antibiotics. Overall, the ability to collect diffraction data at near-physiological temperatures promises to provide new fundamental insights into the structural dynamics of the ribosome and its functional complexes.
About The Speaker
I completed my B.Sc. at Bosphorus University in 2002 and later obtained a Ph.D., in Molecular Biology, Cell Biology and Biochemistry at Brown University in 2007. Before joining the Koc University in August 2019, I was a member of the Biosciences Division at SLAC National Accelerator Laboratory and also affiliated with Non-Periodic Imaging group at Stanford PULSE Institute. My research focuses on structural biology of mutant prokaryotic ribosomes, where I am interested in characterizing the function and dynamics of these mutants, with an eye toward answering questions in structure and dynamics of ribosomes which are resistant to some of today’s commonly-used antibiotics. My current research efforts also include methods development for time-resolved ambient-temperature X-ray crystallography of large and challenging biomacromolecules at 4th-generation light sources like the Linac Coherent Light Source at SLAC.