Photodynamic therapy of cancer and live cell imaging
Photodynamic therapy (PDT) is a developing treatment modality for several cancer types. In PDT, therapeutic action is achieved by the generation of cytotoxic singlet oxygen (SO) upon the irradiation of a photosensitizer. In order to generate SO, common strategy is to incorporate heavy atoms on sensitizers. However, presence of heavy atoms increases the dark toxicity, which is not desired in practical applications. Here, we are introducing a new concept for activatable heavy atom free sensitization of PDT by designing novel orthogonal BODIPY-based photosensitizers. Although PDT of cancer has been considered as a promising therapeutic approach for decades, broader acceptance by the medical community and applicability is hampered due to two major reasons; (i) poor penetration depth of the irradiation light and (ii) hypoxic nature of cancer cells. Herein we are also addressing these two everlasting problems of PDT by the use of gold nanoparticles. In the second part of this talk, development of fluorescent molecular probes for synaptic transmission monitoring and copper detection in live cells will be introduced. Synaptic transmission is an essential process for neuronal communication in the nervous system and it takes place at synapses with the help of synaptic vesicles. We are employing pH-sensitive fluorescent molecular probes to image the neurotransmission. On the other hand, copper, as a result of its redox activity plays significant roles in important biological processes. When the redox activity of copper is misregulated, it can also lead to aberrant generation of reactive oxygen species (ROS). Thus development of copper-selective molecular probes with their unique fluorescence output provides a potentially powerful toolbox that allows for the mapping of labile metal pools and the study of the roles of these dynamic fluxes in different applications.
About The Speaker
Dr. Safacan Kölemen graduated from Chemistry Department at Bilkent University in 2008. He completed his MSc studies at the same department in 2010. He received his PhD in 2014 from Material Science and Nanotechnology department (UNAM) at Bilkent University, where he worked under the supervision of Prof. Engin Umut Akkaya on designing photosensitizers for photodynamic therapy of cancer. Then he joined to Chris Chang’s lab at University of California, Berkeley as a postdoctoral researcher. His current research focuses on development of fluorescent probes for various applications in biological systems.