EventsDr. Hikmet Budak

Genome Editing in Plants

Genome editing (CRISPR) can be used as an engineering tool to develop new plant species with enhanced diseases resistance. It can also be used as a powerful tool for knockout generation, precise modification, multiplex modification, and the activation and repression of target gene(s). In this talk, I will talk about two RNA-guided genome editing and (a) type II CRISPR-Cas9 which is highly efficient in some species, but not in others, and (b) the class II system component Cpf1 (CRISPR from Prevotella and Francisella 1) in place of the more commonly used Cas9 for common genes. I will also talk about the Cas9 efficiency in wheat and compared to other species. There will be discussion on the editing efficiency of Cas9 and two Cpf1 orthologs, AsCpf1 (Acidaminococcus spp. BV3L6) and LbCpf1 (Lachnospiraceae bacterium ND2006) in wheat and barley


About The Speaker


Hikmet Budak received his Ph.D. degree with a major in plant genetics and breeding and with a minor in biometry from the University of Nebraska-Lincoln (UNL), USA, in 2002. He worked as a Coordinator, and led genetics and breeding program at UNL, USA and then returned to Turkey in 2004. In 2016, he returned to Montana State University, USA as Winifred-Asbjornson Endowed Chair. He also serves as Professor at the University of Worcester, UK and at the University of Nebraska-Lincoln, USA.